- All relevant infection control methods are to be utilised.
- Prepare equipment required:
- Refer to Vascular Access clinical skill for cannulation equipment
- Multi-Sample Adapter
- Vacutainer Tubes
- Lab Form
- Red ‘urgent’ biohazard bag
- Explain the procedure to the patient.
- Do not release the tourniquet (it may be necessary to slightly loosen however, if blood-flow is poor).
- Do not flush prior to obtaining the sample as this may dilute the sample.
- Secure the Luer-Lok multi-sample adapter to cannula / bung, and apply tape to support.
- Insert and fill vacutainer tubes in the following order (tubes marked as bold are standard across all ambulances):
Light Blue
| Sodium Citrate; for patients on anticoagulation medications, such as warfarin. Fill the tube to the line |
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Green
| Lithium Heparin |
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Gold*
| Serum Separation Tube, for biochemistry. Draw x2 for chest pain |
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Orange*
| Rapid serum tube for troponins in regional labs |
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Light green*
| Lithium Heparin Plasma with gel. |
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Purple
| EDTA |
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*May or may not be present, depending on your location.
- Gently invert 6-8 times. Do not shake.
- Remove tourniquet.
- Remove the multi-sample adapter and occlude the vein simultaneously (if “bung” not attached).
- Attach syringe, flush and secure as normal.
- Write patient details (Full name, DOB & case number), time and date of collection on tube and lab form in the appropriate area’s (see additional notes). Leave hospital numbers blank.
- Endorse all tubes and form by signing and include AP number.
- Place tubes and completed request form in biohazard bag.
- Handover sample tubes with the patient.